Effect of Fingolimod on GABA, TNFa, SOD and glutathione peroxidase (Gsh px) level in PTZ induced generalized tonic clonic epilepsy in rats
Background: Epilepsy is one of the commonest serious brain disorders, with the highest risk in infants and older age groups. It is has multiple risk factors and a strong genetic predisposition. Objectives: Fingolimod (sphingosine-1-phosphate (S1P) analog) was tested for antiepileptogenic effects in rat model of pentenyle tetrazole (PTZ) induced epilepsy. Methods: twenty four male Wistar albino rats were randomly divided into three groups; control (group A), epileptic non-treated (group B1) in which epilepsy was induced by sub-convulsive doses of PTZ and fingolimod-treated group (group B2) which received 1 mg/kg fingolimod orally once daily for three consecutive months. Results: spontaneous convulsions (SCs) of epileptic rats as regards the frequency, duration and severity have been reduced while GABA, SOD and glutathione peroxidase levels were elevated at the same time tumor necrosis factor alpha (TNFα) was reduced in fingolimod treated group. In addition a reduction of neuronal loss and preservation of the arrangement of pyramidal cells in the hippocampus was also observed in this group. Conclusion:, fingolimod administration demonstrated an antiepileptic effect through significant improvement of the chemical as well as the histological parameters.